【环境讲坛84期】Highly Aligned Nanofibrous Extracellular Matrix for Diverse Tissue Engineering Applications
来源： 发布时间：2018-07-30 点击次数：
报告人： Feng Zhao
Natural extracellular matrix (ECM) derived from cultured human cells can recapitulate the chemical and biological motifs of the ECM found in native tissues. Compared with ECM derived from animal tissues, cell-derived ECM avoids the problem of pathogen transfer and host immunological reactions, thereby holding greater potential in tissue engineering. We have previously created a highly aligned ECM scaffold by decellularizing fibroblast cell sheets that were grown on synthetic nanogratings. The ECM scaffold is composed of aligned uniform nanofibers with a diameter of around 79 nm, which is similar to the size of collagen nanofibers in native tissues. The aligned ECM nanofibers provide directional cues for cell alignment, which allows for seeding human mesenchymal stem cells (hMSCs) on the ECM sheets to fabricate three-dimensional anisotropic tissue constructs, such as vascular grafts. By co-culturing hMSCs and endothelial cells on the ECM scaffold, we have fabricated a prevascularized hMSC sheet containing highly oriented, dense and mature microvessels, which can be further engineered to form 3D hMSC prevascularized tissues for diverse tissue regeneration purposes. This naturally derived, highly aligned, nanofibrous ECM scaffold holds great promise for translational applications.
Dr. Feng Zhao is an associate professor in the Department of Biomedical Engineering at Michigan Technological University. Her research interests are focused mainly on stem cell engineering and cardiovascular tissue engineering. She also develops synthetic and naturally derived nano-biomaterials for tissue engineering applications. Dr. Zhao’s research is currently supported by National Institutes of Health (NIH), National Science Foundation (NSF), and other funding agencies. Her research products have been highlighted by national and international news agencies, newspapers, and scientific publishers including ScienceDaily, RegMedNet, Stem Cells Freak, Kurzweil, Scicasts, Eurekalert, Genetic Literacy Project, The Peninsula, Latest Technology, and Iran Daily.